Dilution Designer — Molecular Biology Bench Toolkit

EXPERIMENT REPORT — DILUTION DESIGNER

Experiment		Date
Researcher		Organism
Compound		Stock
Culture

Dilution Designer Built with passion for science and discovery

📂 Load ⬆ Import CSV

Welcome to Dilution Designer! Add conditions in the left panel → paint them onto the plate → export your protocol. Switch between Plasmid/Induction and Phage Infection with the Experiment type buttons at the top of the left panel, and press Ctrl+K to search everything.

Experiment type

Experiment

Compound / reagent name

Experiment name

Researcher

Organism / notes

Date

Stock solution

ConcentrationnM

Vol available (µL)

Units

Culture / sample

Vol per well (µL)

Total available (µL)

Sample / culture name

Intermediate stock

Compound pipetted directly from stock into each well.

Conditions & controls

Serial-dilution series

Top concentrationnM

Dilution factor

Number of points

Adds N conditions (e.g. 10000, 5000, 2500…) so you can paint a full dose-response gradient in seconds. Existing conditions are kept.

Auto-fill plate

Fill order

Replicates per condition

96-well plate map

ROWS

COLS

Click row/col buttons to exclude. Use the 🚫 Exclude tool to click/drag individual wells. Row/col buttons toggle all wells in that row or column.

Bench reality check

Per-well volumes — each well is independent

Well	Condition	Type	Phage stock	Target	Culture (µL)	Compound (µL)	Pipette from dilution	Final vol (µL)	Pipettable?

Master mix — grouped by condition (+10% overage)

Condition	Type	Wells	Target	Culture total (µL)	Compound total (µL)	Grand total (µL)

Step-by-step bench instructions

Dilution & Molarity Calculator